After studying drama direction at the Janáček Academy of Musical Arts, which he did not finish, he worked as the artistic head of the Brno theatre Polárka. Together with other young theatre artists he helped make Polárka a closely-followed alternative theatre, focusing mostly on auteur work for an audience spectrum as wide as possible. He was also the artistic head of the Divadlo Petra Bezruče and worked regularly with the Reduta theatre in Brno. In 2013, when the artistic head of the Reduta took over the leadership of the Divadlo Na zábradlí in Prague, Mikulášek became a director there. The theatre currently has the following productions by Mikulášek in its repertoire: The V + W Letters, Europeana, Bourgeoisie, The Grey Seventies, The Stranger, The Golden Sixties, Hedonists and Doctor Zhivago. Mikulášek’s directorial approach frequently involves the dramatisation of novels, original scripts and auteur theatre. He works with cutting, detail, musical contrapposto and parallel plots. His other major source of inspiration is fine art, from which he "borrows" an emphasis on mise-en-scène and lighting. A marked feature of his work is the way in which he plays with the meaning of speeches, context and associations on a given theme. He manages to materialise on stage entirely abstract, philosophical subjects, using very simple means.